Transformed bacteria were then propagated through a multi-step selection process and wild type BAC was selected by PCR.
In the multi-step selection process that followed, which involved individual interviews, group interviews, and a job talk, Hua's outstanding performance positioned her in the first place among all applicants for all positions offered by the academy in that year.
In reaching its estimate, AP looks at the unbound delegates who will eventually emerge from a multi-step selection process under way in eight caucus states and attempts to calculate the number of them each candidate will eventually win.
Following a multi-step selection procedure, recombinant BAC clones were identified by DNA sequencing.
As a result, only tumor cells with intrinsic properties that provide advantages in this multi-step selection process will survive and colonize distant sites.
To achieve this goal we performed a multi-step selection of FISH probes comprised of 35 different genomic regions.
Since exhaustive enumeration of all possible combinations of models in a library would be intractable, a multi-step selection process based on metaknowledge, query information and correlation of errors of the ensemble members is employed.
Although both procedures described above simplify certain aspects of the allele replacement reaction, the process still requires the construction of a plasmid replacement vector and is a multi-step selection procedure.
In addition, we evaluated large arrays of segregants, using multi-step selection procedures, first aimed at eliminating the truly inferior strains with simple high-throughput tests and gradually moving the selection to conditions as close as possible to the real industrial conditions.
A multi-step selection strategy was applied, which included statistical filtering, knowledge-based filtering and time series correlation, to identify miRNAs and genes that are associated with the investigated differentiation process.
Of course, it is unlikely that the actual shaping of the genetic code had much in common with this in vitro multi-step selection of RNA aptamers each time beginning with a random sequence (see our response to reviewer 1).
