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Moreover, activating engine thrust requires a complex multi-step protocol, and cannot be accomplished merely by saying, "Engines on".
The constructs were cryopreserved in a vitreous cryoprotectant (CPA) with a multi-step protocol.
This pathway requires the use of multi-step protocol that make more cumbersome and with lower yields than direct labeling.
Polarized optical microscopy images of the nanocomposites showed that improved CNC dispersion and changes to the nanocomposite microstructure were observed when the multi-step protocol was implemented.
To obtain high-quality RNA from GAS cells, we followed a multi-step protocol.
Details of the multi-step protocol and the subsequent experimental validations will be described from here on.
As a disadvantage, it is a multi-step protocol and is more time-consuming and expensive compared to single PCR assays targeting specific prey taxa.
The supernatant was first separated by isoelectric focusing over a pH range of 3 10 using precast first-dimension drystrip 3 10 NL 18 cm (Amersham) following a multi-step protocol for 90,000 Vht (Protean IEF cell, Biorad).
While already a multi-step protocol, PCR itself is a building block for more complex workflows.
This is a multi-step protocol, where the free energy for a given process is first calculated by performing classical MD simulations using a reference potential and then refined using a higher-level method.
We also used a multi-step protocol with insole plantar pressure measurement to simulate the daily walking activity of the subjects with original and newly padded shoes as closely as possible.
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