After being extracted by methanol, the sample was purified based on multi-step preparation, including dehydration with MgSO4/NaCl and cleanup with neutral alumina.
Graphene oxide (GO -based dispersive solid phase extraction (D-SPE) method combined with multi-step preparation has been proposed for the evaluation of trace aflatoxins in proprietary Chinese medicines (PCM).
This strategy represents a straightforward approach to the synthesis of our target compounds given that the donor can be easily obtained in large quantities in contrast to the multi-step preparation of other galactosyl donors.
In view of the complexity of multi-step template preparation and low production, it is imperative to develop simpler template-free methods for the fabrication of magnetic Co nanowires.
As expected, the polyribosome data sets showed higher variance (presumably due to the multi-step sample preparation) and also more genes with extreme fold-changes.
Proteomic methods are largely based on the use of mass spectrometry (MS), a highly specific, effective, and universal technique that does not require complicated multi-step sample preparation.
This was previously a multi step process.
In contrast to the previously used multi-step procedures for the preparation of analogous substituted pyridine or aza-heterocyclic compounds, the synthesis routes for the ligands described here have generally utilized very mild, one-step reactions with readily available heterocyclic precursors.
Comparisons among three different preparations denoted that in the investigated conditions two-step preparations give larger yields of activated solid in comparison with single-step preparation.
The results provide a strong case for synthetic use of multi-step redox cascades in the preparation of nucleotide sugar products.
Additionally, our system also offers an efficient way to UDP-GlcUA, a compound whose synthesis was equally difficult to achieve till now.[ 7] We also believe that synthesis of UDP-Xyl provides a strong case supporting the use of multi-step enzymatic cascades in the preparation of high-value products such as nucleotide sugars.
