Cryopreservation of SLN single-cell suspensions allows for simultaneous phenotypic multi-parameter analyses and minimizes operator dependent variability.
Additionally, this method is particularly useful for simultaneous multi-parameter analyses of small sample volumes and provides a simple and rapid assay for studying innate immunity in zebrafish.
Disadvantages of immunocytochemistry include restrictions regarding multi-parameter analyses.
Fluorochromes are small, chemically inert molecules representing superior markers for multi-parameter analyses.
Specifically, all assays are restricted to fluorophores with infrared emission spectra, and are not suitable for multi-parameter analyses.
Additional multi-parameter analyses at the single-cell and molecular levels suggested to us that CD44 expression positively correlated with Foxp3 expression in thymocytes, the production of IL-10, and Treg activity in splenic CD4+CD25+ T cells.
Most basic confocal microscopes are equipped to cleanly discriminate no more than four fluorophores in a given sample, limiting the utility of this method for co-localization, co-expression, and other multi-parameter analyses.
While costly and time consuming, these instruments facilitate the statistical analysis of complex cellular events and are ideally suited to multi-parameter analyses, particularly of subcellular criteria that require high-quality immunofluorescence imaging.
Multi-parameter analyses distinguished different cortical types, positioning at one extreme medial and orbitofrontal (limbic) cortices, and at the other extreme, lateral (eulaminate) cortices.
Factorial and multi parameter fit analyses were conducted on the FE results to formulate a general parametric equation for the maximum stress concentration factor in terms of the four dimensionless geometric parameters.
Basic types, characteristics and multiformity of periodic-impact motions of the system are achieved through multi-parameter simulation analyses which provide the partition of the parameter space into qualitatively different regions.
