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The multi-locus estimate of spatial differentiation among 14 populations relative to the whole sampled distribution was large (FST = 0.679).
The expansion time of hg U7 in the Near East, Central Asia and South Asia is more consistent with autosomal multi-locus estimates for the genetic separation of these regions during the Terminal Pleistocene74, suggesting a common demographic process, whose origin was unclear previously.
The calculations based on the simulated datasets were focused on generating average, multi-locus estimates of θ.
Using the IM program allowed us a parameterisation of individual migration rates for different loci while theta and divergence time remained multi-locus estimates.
Although the multi-locus estimates benefited from the statistical power of the full information contained in the eight loci the single locus analysis of population differentiation (Table 5) suggested that gene-flow could vary between genome regions as expected in the model of semi-permeable barriers to gene flow [7].
The multi-locus estimates of migration parameters revealed an asymmetric gene flow from B. azoricus to B. puteoserpentis with a mode around 2Nm = 4.3 (Figure 7, Table 4), while the reverse flow from B. puteoserpentis to B. azoricus did not appear to significantly depart from zero.
Theory suggests that multi-locus estimates of population genetic parameters can be robust even when estimated from just five individuals [ 41, 42].
The use of multi-locus estimates of heterozygosity and genetic similarity has been criticized by authors arguing that global heterozygosity-fitness correlations may be driven by certain loci that are physically linked to fitness-affecting loci [ 35, 36, 44].
This parameter is particularly well estimated for allele frequency data and the multi-locus estimation, while it is slightly underestimated for the two haploid loci.
Support values for the STAR phylogeny were estimated using a multi locus bootstrap [ 52] method implemented in the phybase package [ 48].
***TGUMS marker with multi locus amplifications.
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