The placement of the Plushcap within the tanagers has been confirmed by multi-locus DNA sequence data (Yuri and Mindell 2002, K. J. Burns unpubl. data), although its closest relative within the tanagers remains unclear (K. J. Burns unpubl. data).
We describe the development and validation of minimal methylation classifier (MIMIC), combining CpG signature design from genome-wide datasets, multiplex-PCR and detection by single-base extension and MALDI-TOF mass spectrometry, in a novel method to assess multi-locus DNA methylation profiles within routine clinically-applicable assays.
Firstly, the earlier studies may have lower statistical power due to the smaller sample size (n = 55 chicks, 95% CI: 41.79−63.21), the lower-resolution molecular tools (multi-locus DNA fingerprinting) and statistical methods (exclusion-based analysis) employed (see [28]).
To evaluate the evolutionary history of this unique skink, and specifically to test the "relict" and "recent immigrant" hypotheses, we conducted Bayesian relaxed molecular clock divergence dating analyses of an extensive, multi-locus DNA data set including all Plestiodon species from the eastern United States and the Bermudan P. longirostris.
Several classes of molecular markers were developed for PAC species assignment including PCR fingerprints, single-copy restriction fragment length polymorphisms (RFLP), multi-locus DNA sequences, and microsatellites.
I introduce a new approximate-Bayesian method for comparative phylogeographical model-choice that estimates the temporal distribution of divergences across taxa from multi-locus DNA sequence data.
We assessed the genetic similarity of mates using band-sharing coefficients derived from multi-locus DNA fingerprints [ 29], following standard laboratory and scoring procedures (e.g. [ 8]).
An extension of the supervised classification procedure is planned as future work, where the issue of specimen to species assignments with multi-locus DNA Barcode sequences will be analyzed and addressed.
Multi-locus DNA sequence estimates of North American Limenitis relationships posit that the mimetic L. a. arizonensis diverged from a lineage which eventually gave rise to the mimetic L. a. astyanax and the non-mimetic L. a. arthemis [ 4, 18].
Technical advancements now provide the opportunity to address questions about ISEA history by extending beyond mtDNA and NRY analyses to that of multi-locus autosomal DNA.
For example, within the spruce genera morphological and molecular markers often give conflicting results and multi-locus nuclear DNA data indicates that incomplete lineage sorting is a major challenge for inferring species trees [ 33].
