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On the other hand, the analyses of multi-locus datasets are more challenging.

Our simulations demonstrated clearly the utility of multi-locus datasets in estimating population genetic parameters under a coalescent framework, validating a key component of theoretical investigations of optimal sampling strategies [14], [17].

Future phylogenetic studies based on large, multi-locus datasets that utilize a species tree framework could potentially help to resolve these problematic areas of the OPM phylogeny [ 12].

When ND2 was analyzed separately (tree not shown), its topology was identical to that provided by the combined cytB and ND2 results (Fig. 3), while the topology for cytB alone differed from results given by the multi-locus datasets.

Multi-locus datasets are created from individual alignments that do not have "too many" missing entries using a bipartite graph of taxa and loci and combining with bicliques or quasi-bicliques [ 22, 23].

We propose a dual approach consisting of high-throughput genomic data to resolve the two most difficult phylogenetic problems (regions A and B) and the development of targeted multi-locus datasets of to resolve the remaining problems in the Rhodymeniophycidae (regions C-E).

However, since the variance in genetic diversity at a single or a few loci is unlikely to reflect the overall genomic patterns, assessment of the proportional contribution of drift, selection and demography in shaping genetic variability and population differentiation should ideally be based on multi-locus datasets [ 7, 9, 10].

Second, the relationships in regions C, D and E require generating large multi-locus datasets for a broad selection of Rhodymeniophycideae for which targeted PCR amplification may be preferable to high-throughput genomics because of the large number of taxa involved and lower estimated data requirements.

The same topology was found in both MP and Bayesian analyses irrespective of utilizing codon positions for the Bayesian cytB analyses and also for each of the two multi-locus datasets; however, the mixed models provided increased support indices at most nodes for all data sets, and therefore, only the support indices while utilizing codon partitions are shown for the Bayesian results (Figs. 2, 3).

Pairwise FST for the multi-locus dataset are reported in Table 2. Overall the nuclear dataset measured lower levels of population structure compared to mtDNA.

In the multi-locus dataset including the CR (Fig. 3B), G. i. tenuirostris and G. coprotheres are posited as sisters with only weak statistical support.

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