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Geometrical properties of the microstructure of whole bovine cervical mucus were studied.
The rheological properties of mucus were studied with a microrheometer, and in vitro mucociliary transport (MCT) (frog palate), contact angle (CA) and cough clearance (CC) (simulated cough machine) were measured.
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The effects of DSS on mucus permeability were studied using the RC-50 imaging chamber mounted onto an upright confocal microscope.
25 When such animals were studied, an inner mucus layer was observed, but it was penetrated by bacteria, and massive infiltration of immune cells was observed (figures 1 and 2C, Tlr5−/− Infl).
Importantly, this system allows components of the innate immune system, including mucus physiology, to be studied in isolation from the adaptive immune system.
The intestinal mucus adhesion or penetration of liposomes was studied by confocal laser scanning microcopy and fluorophotometry using coumarin 6 as the fluorescent probe.
Although the effector mechanisms responsible for the resistance need to be studied further, changes in goblet cell populations and increased expression of terminal Fuc residues in intestinal mucus appear to be involved in resistance against E. caproni.
No other methods to eliminate mucus were evaluated in the present study.
However, in this study the properties and in vitro transportability of mucus were not determined.
The microbiota mucus sample strategy used may have reduced the observed differences in mucus microbiota as the amounts of attached mucus were not controlled for.
Mucus-liposomes interactions were also studied using cell cultures, Caco-2 (without mucus) and cocultures Caco-2/HT29-MTX (mucus producing).
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