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Clinical symptoms and a subjective assessment of stool features (consistency, frequency, presence of blood of mucus) were recorded.
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Nanocomplex movement in mucus was recorded using multiple particle tracking (MPT) method.
Stools emissions (including number of emissions/day), with mucus and/or blood, were recorded and consistency of each stool was assessed using the 7-point Bristol Stool Scale (type 1 corresponds to separate hard lumps, like nuts while type 7 corresponds to watery, no solid pieces, entirely liquid) [ 11, 12].
Secondly, green fluorescent beads with a diameter of 2 µm were allowed to sediment onto the mucus surface, and confocal XY stacks were recorded directly and after 15 min incubation with 3% DSS or 3% Dextran (Fig. 1C).
Rectal temperature (T), heart rate (HR), respiratory rate (RR), mucus membrane color, indirect systolic (S), diastolic (D) and mean (M) arterial blood pressure (BP) were recorded prior to premedication and again prior to anesthesia induction.
Axial Z stacks (0.5 μM) were recorded of representative areas to construct a three dimensional virtual images of the tissue, overlying mucus and biofilm.
Quality of mucus (normal or purulent) was recorded.
Production of cervical mucus as assessed by the Billings Ovulation Method of Natural Family Planning (NFP) (Billings et al., 1974) was recorded daily.
No differences in the amount of loose mucus were detected.
The microbiota mucus sample strategy used may have reduced the observed differences in mucus microbiota as the amounts of attached mucus were not controlled for.
Sometimes mucus is produced.
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