Mucus penetrability phenotypes were again different as a more penetrable inner mucus layer was observed in mice colonized with microbiota from Room 2, while mice colonized with microbiota from Room 1 showed a mucus layer impervious to beads (Fig 5B and C).
As regards follicle size, the highest viability and penetrability were obtained with oocytes from follicles >2.20 mm (P>0.05).
Therefore, the increased membrane penetrability is the direct evidence of cell membrane damage.
When provoked, this ribbon worm releases substantial amounts of mucus; this mucus was collected and lyophilized.
Mucus penetrability, quantified as the number of beads close to the epithelium (20 µm into the tissue and 120 µm into the lumen), was ∼40% in patients with active disease, 10% in patients in remission, and close to zero in the controls (figure 5B).
To quantify mucus penetrability, we estimated the bead intensity in close proximity to the epithelium (figure 4B).
In more sophisticated modern techniques the radius value is taken from other experiments, and alpha-decay data and penetrabilities are used to calculate the frequency factor.
Dr. Fry did not find any venomlike proteins in the mucus, but later he realized mucus was the wrong place to look.
Mucus quality was graded in a scale of 0 to 3 (0- no mucus is evident; 1- transparent mucus is present; 2- colored mucus present; 3- abundant and colored mucus is present).
