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These gastropod groups constantly produce mucus, for example to aid locomotion [ 27, 28] or to attach to substrates [ 29], thus providing a generally humid environment favorable for the nematodes.
One might speculate that the "extra" components in the sputum confer different biological properties to mucus; for example, the role of plasma-derived proteins as complementary factors to epithelium-derived proteins in the physiology of normal mucus is likely to be important.
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If the production of mucus is reduced (for example, due to goblet cell damage, age or hormonal status), [ 1] mucus distribution over the preocular surface is impaired, leading to poor contact of the tear film with the eye surface and a loss of film stability [ 4]. Figure 1 shows the location and extent of epithelial mucins on the ocular surface in a healthy eye compared with a severe dry eye.
In mice with a thinner or no functional mucus layer, as for example germ-free mice, DSS treatment induces an acute and massive bleeding long before any inflammation is observed [20], [25], [26].
However, mucus thickness alone was not a useful indicator of mucus barrier function since, for example, the IL-10-deficient mice had a thicker inner attached mucus layer.
For example, mucus in the lungs traps potentially harmful pathogens so that the cilia can move them away before they can infect the cells (Knowles and Boucher, 2002).
For example, plants often have very specific mechanisms to prevent fungal pathogens from entering leaf tissue [ 6] and some species produce layers upon their epithelium - the first barrier against infection - to obstruct parasite penetration (for example, mucus in coral protection [ 7] or salivary mucins to preserve the oral cavity health [ 8]).
62, 63 For example, mucus-associated bacteria are believed to regulate the settlement and/or growth of opportunist microbes by occupying space or producing effective antibiotics.
Tissue damage, inflammation and disease-specific changes in-host phenotype (for example, increased mucus volume and adhesiveness in CF lungs, Boucher, 2007) are accompanied by changes in the growth substrates available to bacteria.
Cilia are motile, whip-like structures that protrude from cells and move fluid across the surface of a tissue, for example sweeping mucus and dirt out of the lungs, and driving movement of an egg from the ovary to the uterus.
We excluded aetiologies in which response to treatment could be affected by complicating factors, for example, poor mucus clearance attributable to primary ciliary dyskinesia, allergic inflammation and immune deficiency.
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