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The objective of this study was to develop an accessible 3D printed device that would allow manipulation of the gas mixture used to supply the tissue culture media separately from the gas mixture exposed to the mucosal side of explants.
PNA was a targeting moiety that binds to β-d-galactosyl- 1-3 -N-acetyl-d-galactosamine, which is the terminal sugar of the Thomsen–Friedenreich antigen that is specifically expressed on the mucosal side of colorectal cancer cells.
PNA is a targeting moiety that binds to β-d-galactosyl(1 3 -N-acetyl-d-galactosamine, which is the terminal sugar of the Thomsen-Friedenreich antigen that is specifically expressed on the mucosal side of colorectal cancer cells.
Peanut agglutinin (PNA) was immobilized on the surface of fluorescent nanospheres through a chemical reaction with PMAA in order to recognize β-d-galactosyl- 1-3 -N-acetyl-d-galactosamine (Gal-β-d-galactosyl- 1-3 -N-acetyl-d-galactosamine the Thomsen–Friedenreich antigen that is specifically expressed on the mucosal side of colorectal cancer cells.
PNA is a targeting moiety that binds to β-d-galactosyl- 1-3 -N-acetyl-d-galactosamine (Gal-β-d-galactosyl- 1-3 -N-acetyl-d-galactosamine the Thomsen–Friedenreich antigen that is specifically expressed on the mucosal side of colorectal cancer cells; it is anchored on the nanosphere surface via a poly(methacrylic) acid (PMAA) linker.
The basal electrical parameters were not altered when concentrations up to 8,000 LD50/mL were incubated in vitro during 60 minutes in the mucosal side of the intestinal tissues (Fig. 2A and 2B).
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Everted gut sac analysis revealed that the absorption of nSP70-C and nSP70-N from the mucosal side to the serosal side of the sacs was significantly greater than the absorption of the other silica particles after incubation for 45 min.
HRP and 51Cr-EDTA were added to the mucosal side to a final concentration of 10−5 M and 34 µCi/ml, respectively.
Here, CLm-i, CLi-m and CLi-s were transport clearances of peptide from the mucosal side to the intestinal tissue, from the intestinal tissue to the mucosal side, from the intestinal tissue to the serosal side, respectively.
Basal electrical parameters of ileal segments of rats mounted in Ussing-type chambers were unaffected by concentrations of toxin up to 8,000 LD50/mL incubated in the mucosal side for at least 60 minutes.
On the other hand, when the remaining activity was estimated from the elimination clearance from the mucosal side, the overall transport of CcpLE was much higher (6.95 μl/min/cm) than that of CcpLEamide.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com