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Further studies on expression and regulation of STAT4 in the intestinal mucosa will be required to investigate the functional consequences of STAT4 gene variants in more detail.
At surgical resection blocks of tumour and normal mucosa will be also be collected.
At each visit the entire oral mucosa will be examined under WL, with surgery sites photographed.
A further six month review is scheduled when the clinical appearance of the oral mucosa will be re-evaluated.
In nasal secretion the presence of antimicrobial peptides and pro-inflammatory mediators will be measured and the microbial composition of the nasal mucosa will be analysed.
The origin in the airway epithelium indicates that the total surface area of the airway mucosa will be an important determinant for FeNO [ 7].
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Secondarily, B lymphocytes will secrete IgA into the mucosa that will be specifically directed against invasive pathogens [ 29– 31].
As biological samplings will go on for some days, the samples for some of the children absent from school on the established day for taking oral mucosa cells will be retrieved during the whole period of biological sampling.
Tumor and normal mucosa samples will be collected only in patients enrolled in the four-arm phase II study: at baseline (possibly within the diagnostic rectal biopsy) and at surgery for all patients and at day -4 in patients assigned VPA.
The mucosa and submucosa will be removed (1 2 mm) around the internal opening.
By taking Buccal mucosa swabs, we will be able to study if there are responder differences caused by the Apo-E4 status.
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