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The carcinoma tissue and the corresponding normal mucosa were fixed with 80% ethanol and embedded in paraffin.
Endoscopic biopsy samples of the gastric mucosa were fixed in 10% formalin and embedded in paraffin wax, and 4-μm-sections were stained with hematoxylin-eosin for routine histology.
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Following the fluorescence observation, the colonic mucosa was fixed and histological examination was independently performed.
Selected sections were stained with PAS-reaction For transmission electron microscopy skin and oral mucosa tissue were fixed in 2.5% glutaraldehyde/cacodylate buffer for 24 h, post-fixed in 1% osmium tetroxide, dehydrated in a graded series of alcohol and embedded in epon (Serva, Heidelberg, Germany).
Finally, the intestinal mucosa samples were fixed in 10% formaldehyde solution at room temperature according to standard method.
Carcinomas with adjacent mucosa tissue were fixed and embedded in paraffin, and sectioned for staining with H&E.
Normal oral mucosa and tumour tissues were fixed with 10% buffered formalin embedded in paraffin and decalcified in 10% EDTA solution.
For transmission electron microscopy (TEM), 1.0 mm2 samples from the mucosa of the large intestine were fixed in 2.5% glutaraldehyde in phosphate buffer.
To test the tissue permeability of fixatives, the maxillae were fixed in acetone, glutaraldehyde, and PFA with or without removal of palatine mucosa before fixation.
After the culture period, explants were fixed in formalin and H&E stained sections were evaluated for histological defects of the mucosa.
The 77 clinical samples of cancers and adjacent normal mucosa and the resected xenografts were immediately embedded in OCT compound (Sakura Finetek Japan, Tokyo, Japan) for immunohistochemistry. Frozen sections were fixed with 4% paraformaldehyde at room temperature (RT) for 10 min.
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