The top 5% of the corrected nasal mucosa values derived from the nasal mucosa atlas were excluded to avoid error, and then the threshold for enhancement was set to be 25% of this remaining maximum value.
The wound was inflicted using a 2 mm punch biopsy (Miltex, York, Inc. PA, USA) in the buccal mucosa anatomical site taking caution to avoid rupturing any major vessels by trans-illuminating the surgical site.
It should be kept in mind that we used suboptimal concentrations of all antibodies for the staining of colonic mucosa and colorectal cancer tissue to avoid unspecific staining.
Close contact was maintained with the rectal mucosa, but care was taken to avoid applying excessive pressure to the rectal wall with the ultrasound probe to minimize any distortion of the ultrasonographic and Doppler signals owing to arterial occlusion or compression.
Moreover, in sheep, Derwall et al. [ 23] demonstrated that during administration of gaseous H2S via an extra-corporeal, veno-arterial membrane oxygenator to avoid any airway mucosa damage related to the gas inhalation [ 24], [ 25], whole body O2 uptake, CO2 production, and cardiac output remained within the physiological range.
The enzyme concentration and the exposure time were kept low to avoid damage to colon mucosa.
In the present series, biopsies were taken from the middle-thoracic esophagus to avoid sampling severely inflamed mucosa.
Standard of care ventilator support requires the delivery of humidified and heated air to patients in order to avoid drying the airway mucosa [ 60, 62].
Here, stringency level II (Additional file 2: Table S2) was used to calculate probe-specific thresholds to avoid hypermethylation in normal mucosa.
The three buttons must be slid in turn to avoid laceration of esophageal mucosa, severe ache or huge hemorrhage and ensure a safe process of capsule placement.
