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Second, we observed mucosa samples of enteritis rats to get CMDI and HS scores (The DAI, CMDI and HS scores were shown in Tab. 2).
In that seminal study, hypermethylation of the MGMT gene was observed in 46% of tumors as well as in 50% of normal appearing colorectal mucosa samples of patients in whom MGMT promoter methylation was found in the corresponding tumor.
Therefore, the aim of this study was to evaluate methylation patterns of genes involved in colorectal carcinogenesis through this mechanism in both tumor tissue and normal appearing colorectal mucosa samples of patients with multiple and solitary CRC, as a proof-of-concept of a putative underlying epigenetic defect associated with tumor multiplicity.
Earlier studies demonstrated Th17 stimulation, IL17 related mRNA expression, and IL17 production in inflamed mucosa samples of CD and UC patients [ 10, 22– 22].
Both experimental groups showed significantly lower mRNA abundances of several pro-inflammatory genes in mucosa samples of the three parts of the intestine than the control group.
We constructed a dataset containing the 16S rRNA sequence data obtained from the analysis of the ileum mucosa samples of four unrelated children: two patients with inflammatory bowel disease and two sex- and age-matched healthy controls.
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When we studied the methylation status of some of the CpGs found to be mutated in the tumor of Patient 1 in a colonic mucosa sample of a normal individual, cytosines in all the investigated CpG sites were found to be methylated.
Of note, four CpG sites found mutated in APC exon 6 and MLH1 exons 13 and 18 were analyzed exemplarily by bisulfite genomic sequencing and were all found to be methylated in the colonic mucosa sample of a normal control (Supplemental Fig. 1A) (see Section 4).
Briefly, adults with asthma, allergic rhinitis or no underlying respiratory disease were recruited; after the onset of 'common cold' or ARI symptoms they visited the study clinic for nasal mucosa sampling on D2 and D6 of symptomatic illness, and an asymptomatic BL sample was taken at least 29 days later.
The integrated prototype endocytoscopy has the potential to assess the correlation between the area of mucosa observed and the area of mucosa sampled for histopathology, and a suction mark was made via the suction channel of the scope, immediately after the affected lesions were observed using the endocytoscope.
Nevertheless, integrated prototype endocytoscopy has the potential to assess a pinpoint correlation between the area of mucosa observed and the area of mucosa sampled for histopathology.
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