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Some melanomas had much weaker staining.
In OA tissues, TWEAK expression was confined mainly to the lining region with much weaker staining intensity compared to active RA synovial tissue.
Cells of the resting and proliferating zones (that is, areas of proliferation and matrix synthesis) showed no or very much weaker staining (Fig. 3e).
After 60 min the intensity of the staining had decreased, but the pattern with more intense staining over Kupffer cells and much weaker staining over other cells remained (not shown).
A marked reactivity for the protein was observed in tumour cells of 61 out of 61 cases of papillary carcinoma; a much weaker staining was present in the rim of peritumoral normal follicles with tall epithelium, whereas normal follicles with flat epithelium were not stained.
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In the liver tissues of control mice, extensive fibrosis displaying a honeycomb pattern of fibrous septa (blue staining) was evident whereas much weaker collagen staining was found in the livers of PP2Ac α knockout mice.
In mock transfected HEK 293T cells, a main nuclear and much weaker cytosolic staining was observed (Figure 4A).
However, we noticed that one of the four centrioles often showed much weaker CP110 staining.
The weak single-line labeling outside of aggregates and the much weaker cilia staining (AcTub) in NPHP RNAi) animals suggested general lumen defects.
L243 also stained JN spots and the cell surface, but this staining was much weaker than the staining of CFs.
TgICMAP1 is likely to have a high affinity for MTs, as heavy eGFP-TgICMAP1 coating correlated with much weaker anti-tubulin staining when compared with that of uncoated MT segments, suggesting that perhaps TgICMAP1 binding blocks the accessibility of tubulin antibody to the MTs (Figure 2, insets).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com