However, in fezf2 mRNA-injected embryos, a much stronger nuclear accumulation of β-catenin was found throughout the embryo, suggesting widespread hyperactivity of Wnt/β-catenin signalling (supplementary material Fig. S5Nb,b′).
The observed stronger suppression of all AtPHT1 transcripts in the roots upon Pi re-supply could be due to a systemic signal, given that the nutrient solution is quickly depleted of Pi and Pi accumulation is much stronger in the shoot than in the root itself (Jost et al., personal communication).
In our experiments the inhibition of monocyte recruitment to the subretinal space in Cx3cr1 −/− mice (Ccl2, Ccr2 deletion and monocyte depletion, Figs 3 and 5) resulted in a 50% reduction of subretinal MP accumulation, but in a much stronger reduction of photoreceptor cell death (Figs 4 and 5).
Interestingly, upon the expression of T59A or T59D mutants, the accumulation of jBid was observed, which was much stronger in the case of T59D mutant mimicking constitutive phosphorylation.
Visualization of the brain accumulation by near-infrared imaging revealed a much stronger disruption of the BBB, mainly around the eyes, in the vehicle group as compared to R-flurbiprofen treated mice (Fig 10A and B).
Its accumulation started within 2 h after treatment and became much stronger over time.
Therefore, it can be concluded that at the protein accumulation level, the impact of the gln1.3 mutation was much stronger than that of gln1.4.
There was only a weak correlation between 137Cs and C accumulation above 15 cm soil depth, but, in contrast, a much stronger relationship was found below 15 cm.
This is consistent with the much stronger correlation of outdoor UFPs with coarse PM (r = 0.83) than with accumulation-mode PM (r = 0.44) [Supplemental Material, Table 1 (available online at http://www.ehponline.org/members/2008/11189/suppl.pdf)].
We also observed a much stronger band at 22 kDa and a band at 15 kDa that was not seen in the wild type, indicating accumulation of proSP-CA116D forms.
