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Exact(6)
Our identification of three additional Rickettsia T4SS components (VirB1, VirB2, VirB7), all of which comprise much shorter sequences than the more conserved components (Table 4), implies that short (under 250 aa) or hyper-variable components are not easily revealed by automated methods.
No DEG7 orthologues were found in animals, since all hits have much shorter sequences and no hit contained a second protease domain (query coverage <30%; results not shown).
In sum, the long-standing lack of full resolution within Ursidae may be primarily due to the low level of variation harbored in much shorter sequences than those used in this study.
The fact that higher improvements are obtained for shorter sequences was confirmed through the simulation of 1000 G ML families inferred from much shorter sequences (400 bp), where still a higher improvements where obtained (see Table 2).
Compared with standard approaches that infer selection coefficients from the size of dips in genetic diversity around the adaptive site, our estimator requires much shorter sequences but sampled at high population depth to capture low-frequency variants; given such data, it consistently outperforms standard approaches.
The new method, "SeqZip", uses oligo ligation directed by the mRNA template to report on exon-exon joints present in the mRNA and to condense distant regions of splice variation into a much shorter sequences assayable by sequencing or simply by size.
Similar(54)
Since BF-15 retains most part of the antimicrobial activity of BF-30 but has significantly reduced haemolytic activity and a much shorter sequence length (and less cost), it is a particularly attractive template around which to design novel antimicrobial peptides.
To more specifically investigate binding of SF2/ASF to the predicted MTRR ESE motifs we performed RNA affinity chromatography with RNA oligonucleotides that harbor the wild-type and mutant ESE motifs as part of a much shorter sequence.
However, despite the fact that frequent inclusion of exon 12a may imply only infrequent translation of exon 12b, the ancestral coding meaning of exon 12b has been highly conserved in the vast majority of vertebrate Meis genes; the only exceptions are anole lizard Meis3, which seems to have lost the entire exon, and zebrafish Meis1.2, which has a much shorter sequence.
2) By considering larger n-grams, miRCluster is developed to treat much shorter mature sequences.
While a long nonrelated sequence at the 3′-end delayed but did not prevent establishment of the productive infectious cycle, a much shorter extra sequence at the 5′-end completely abrogated virus replication.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com