The C-terminal quarter of the protein, while always highly charged, shows much more sequence diversity.
The remaining, mostly-Eurasian Elymus sequences form a very weak (28% BS) group with much greater sequence diversity.
The translated SplB (BPUM_1283) sequence is highly conserved in B. pumilus, but SplA (BPUM_1282) shows much more sequence diversity among B. pumilus, B. subtilis, and B. licheniformis (Table 2), indicating possible differences in SP lyase genetic regulation.
B. pumilus SodA is highly conserved with respect to the homologs of B. subtilis and B. licheniformis, but there is much greater sequence diversity in SodF and YojM (Table 3).
The Tp2 alleles in parasites isolated from cattle that shared grazing with buffalo at a single farm in the Rift Valley of Kenya showed much greater sequence diversity than those from cattle where buffalo were not present, although these were derived from geographically distant sites.
As to how much their sequence diversity affects their function during T-DNA transfer remains unknown.
Compared to the three previously sequenced pea Ogre elements [ 21], this set of Ogre-like contigs showed much higher sequence diversity, suggesting they represent several distinct Ogre subfamilies.
This assessment, initially reported for large scale human genotyping, has been corroborated in plant species including autogamous crops with low nucleotide diversity (0.2% to 0.5%) [ 3, 27- 29] and outbred species with much higher sequence diversity typically ≥2% [ 9- 13].
But this begs the question of why duplicated tRNAs in yeast retain nearly 100% sequence identity - one would expect, given the data presented here, to see much more sequence diversity.
