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As can be seen in Figure 4 there is a qualitative and quantitative difference between non-specific binding to the two surfaces with a much reduced binding to the GLISS surface.
In accordance with the data from the on-array streptavidin-binding assay, mutant C12G23 is structurally distinct from St-2-1 and has a much reduced binding affinity, whereas mutants A12T23 and G12C23 are structurally similar to St-2-1 and retain a similar binding affinity (DNA structures from http://mfold.rna.albany.edu/?q=mfold/dna-folding-form).
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These mutants showed much reduced actin binding, although they did bind to actin significantly better than the polypeptide lacking the entire dimerisation domain (Table I), suggesting that the dimerisation domain may also interact with actin.
However, unlike the abrupt onset of knock-on damage in bulk materials, this onset is continuous and occurs at much lower energies because of the distribution of reduced binding energies of atoms at surfaces, interfaces, or defects [24, 25].
One deficit is reduced binding to BHK-21 fibroblasts [20].
All trinucleotide changes significantly reduced binding affinity.
Soluble pegloticase reduced binding by 70%.
A non-consequential Leu157 to Ala (L157A) mutation that displayed much reduced foci formation and TfR binding had normal TfR distribution, normal surface TfR level and normal Tf internalization.
The amount of nonspecific binding proteins on 6a 6b was much reduced compared to that on commercially available poly methacrylate) resins, Toyopearl™ (8), and was almost the same as that on one of the most popular resins, Affigel™ (9).
By contrast, BI1003, which showed potent inhibitory effect on the transactivation of RXRα and RXRα-F313A by binding to their LBP, had much reduced inhibitory effect on the constitutive transactivation of both mutants.
Furthermore, the shifts induced by a truncated IgFLNa20 protein (residues 2167 2235), which lacks the residues forming the first β-strand, were very much reduced, demonstrating that this region of IgFLNa20 is required for binding IgFLNa21.
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