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For example, Figure 5 shows the GMS profile of a 1000 bp region of human chromosome X using 50 bp (red) and 100 bp (blue) reads and shows much more of the region can be unambiguously mapped with 100 bp reads compared to 50 bp reads.
We focus on ESTs rather than NGS data here primarily because the much longer EST sequences are largely mapped unambiguously to the genome and are much more likely to contain splice junctions when they originate from spliced transcripts than the much shorted NGS reads.
Seen in this light, their description of the carbamoyltransferase dataset is useful, but I think it might help if the authors were to address the relevance of this single example of paralogous gene loss more explicitly and in more detail; i.e. how much more of the data do they think can be explained unambiguously as losses?
In contrast, the domain architectures of these motors have been much more extensively modified during diversification of lineages, such that only 3 can be unambiguously traced back to the LCEA.
Most notably is that the long reads produced by 454 allow more tags to be mapped unambiguously to a gene as compared to SAGE where the short ESTs are much more likely to align to multiple loci.
The Democratic Party is also more unambiguously progressive.
So try again, and do it more unambiguously and emphatically this time.
A backlash could help more Democrats get elected, and push elected Democrats to more unambiguously support legalization.
It is much, much more.
Aspirations towards landscape should be defined more unambiguously so that they can be assessed alike.
He was more, much more".
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CEO of Professional Science Editing for Scientists @ prosciediting.com