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The results of the qRT-PCR analysis indicated that in ZH11C plants, the highest transcript levels of GNS4 were in the young panicle, with much lower transcript levels in the stem, leaf, and leaf sheath (Fig. 6a).
Specific NF-Y genes such as Solyc12g027650 were more abundantly expressed in buds and flowers, but much lower transcript levels in fruit.
However, the much smaller ZCCT2 transcription (due to its strong repression by the various compounds tested) in Tsp compared to Ch was associated with much greater VRN1 expression and much lower transcript levels of the genes related to freezing tolerance.
OsCSLF2 and OsCSLF7 show a uniquely high co-expression pattern with OsCESA4, -7 & -9 in the hull/spikelet tissue typical of secondary cell walls; however, they both have a much lower transcript level than OsCSLF6 and OsCSLF8.
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First, the FPKM cumulative distribution revealed that these novel NTRs had much lower transcription levels than known transcripts (genes).
Importantly, the steady-state levels of the two terminal enzyme transcripts (BAPT and DBTNBT) are much lower than transcripts of early pathway steps.
At all three loci, both sense and antisense transcripts can be detected, albeit with antisense transcripts at much lower level than the sense transcript.
First, the transcripts could not be picked up in other tissues due to either polymorphic nature of SSRs or much lower number of transcripts, and second, they are transcriptionally dormant in other tissues barring testis/spermatozoa.
Thus, the average higher FA/MA value for OBPs in RpL8 normalized cDNAs (1.65) compared to OR7 normalized cDNAs (1.17) might represent an artifact due to a much lower level of OR7 transcript in corresponding male sample.
The second group was up-regulated at 27 DAP and is characterized by much lower relative levels of transcript accumulation.
Therefore, the number of actual unique genes is expected to be much lower than the total transcript sequences coming out of any de novo assembling tool.
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