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Patients with clinical benefit had a tendency to increase VEGF-A levels at a much lower fold ratio than patients with disease progression.
Although this is a much lower fold difference than seen with rIL-1β it may indicate the acute phase response is retarded when cells are stimulated with rIFN-γ compared to rIL-1β.
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However, the array data displayed much lower fold-change values compared to the PCR, as displayed in Figure 2C.
Both cytokines were still up-regulated at T24 in comparison to mock-stimulated cells but with a much lower fold-change than at T4 (Table 5).
Much lower (0.1 fold TBP) was the expression of IL-22R followed by even lower amounts of IL-20R1 suggesthat that STAT3 activation of cultured keratinocytes is mediated by engagement of the type II receptor IL-22R/IL-20R2.
In contrast, HDAC1 and HDAC2 mRNA correlated more closely with protein expression: HDAC1 mRNA levels were much lower (5-15 fold) in most differentiated tissues than in mESCs, whereas HDAC2 mRNA levels were similar in mESCs and differentiated tissues.
Our results reveal significant differences in magnitude between the ≈300-fold change in transcript expression and the much lower ≈8-fold-changes in FGF19 protein.
As to another starch-degrading gene amyA2 (CAP0168), no obvious changes were found in the expression level at the first three phases, and only a 2.8-fold increase occurred at S phase (Additional file 2), which was much lower than fold-changes of other starch-degrading genes.
Interestingly, in the case of PelII from X. campestris[ 28], the K m value was reported to be only 0.98 g L−1, which was 3.6-fold lower than that of PelN, while the kcat value of PelII was much lower (10-fold) than that of PelN.
Variability in the expression of Rho-GDI and ERK2 proteins was much
Notably, expression of ERRγ was much lower (0.3-fold) in SC than in VIS adipose tissue.
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