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An immature HIV particle contains approximately 1500 copies of p24, while other HIV proteins are present at much lower copy numbers [54].
The ploidy of the third plasmid, pHS1, is regulated similar to that of the chromosome, but it has a much lower copy number in early exponential phase.
While Gag proteins are highly abundant, with a copy number of
Nuclear mitochondrial insertions (numts) could be ruled out for long range PCR by joining the overlaps creating a circular sequence, and are not expected to be a problem for hybridization capture since nuclear DNA has a much lower copy number than mitochondrial DNA.
However, a few sequences showed a much lower copy number than that estimated by RECON.
Genome-wide studies in E. coli revealed that some proteins can be expressed in much lower copy numbers than 60 [ 3].
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glp-4 mutants lack a gonad and have much lower genome copy number and reduced variance compared to wild-type worms.
The much lower TIR-220 copy number in the D. americana assembled genome is probably a consequence of the shorter read lengths produced by next generation sequencers, what poses even bigger challenges to the assemblage of repetitive regions.
The SOAPdenovo2 assembly also had a much lower amount of copy number errors, but did have more substitution errors [ 1].
In this study, however, the viral load in breast carcinoma was much lower than 1 copy per cell.
Within an infected host, HIV-1 proviral copy number is typically much lower than even single-copy nuclear genes; HIV-1 DNA is thus harder to PCR-amplify.
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