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By engineering two copies of the tet operators flanking the TATA box of the human U6 promoter, we created a U6 promoter derivative (2O2) that exhibited much lower basal transcriptional activity compared with recently reported inducible pol III dependent promoters.
Interestingly, the SA content was in a much lower basal level in the mutant than in Wangshuibai, implying that the mutant have SA synthesis deficiency.
In gamergates, which have a much lower basal catalase activity, the reduction was only slight but also highly significant (statistics, see above, t = 8.37, p<0.001).
Only the probe sets for Lbx1 and its corepressor, Lbxcor1, showed significant signal reductions in the white cell comparison, albeit from much lower basal signals than in the green cell comparison.
When equal numbers of live bax +/+ and bax -/- cells were seeded and mock-treated, bax -/- cells produced more colonies, consistent with the much lower basal level of cell death in these cultures (see Figure 4).
In contrast, CLE209 was only induced about 5-fold over a much lower basal expression level relative to CLE210 such that CLE210 mRNA was ~37-fold more abundant than CLE209 at day 2 but only ~6-fold more abundant at day 10.
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The 50%-growth inhibitory concentrations (IC50s) of dasatinib were much lower in two basal B cell lines than those in the other cell lines.
Following treatment with LY294002, a dose-dependent reduction in BCL-2 promoter activity was observed only in the MIA-PaCa-2 cell line, but not in the PANC-1 cell line, which has a much lower level of basal AKT activation.
Although TMX2-28 cells overexpress CKs 5, 14, and 17 compared to MCF-7 cells it is important to note that TMX2-28 cexpresspress much lower levels of these basal CKs than do the 184 HMECs, which display a basal phenotype.
p53 mRNA was at high levels in the hair follicle and basal layer of the skin, EGL of the cerebellum and intestinal crypts, which are actively proliferating; in contrast, p53 mRNAs were at much lower levels in supra basal layer of the skin, internal granular layer (IGL) of the cerebellum and villus of the small intestine, which belong to the differentiated compartments.
At 24 h, the ROS level was much lower than that of the basal level.
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