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Compared with the linear poly(AM-co-AMPS) having the similar molecular weight, S-poly(AM-co-AMPS) provides the drilling fluids with better shear-thinning characteristics and much lower apparent viscosities under non-aging and high-temperatures.
While the high apparent flow activation energies in dynamic shear and capillary rheometry can be explained by simple thermal degradation, melt viscosities interpreted from the instrumented batch mixer showed a much lower apparent activation energy (186 kJ/mol).
Ligand depletion may also account for the discrepancies observed between the results reported by Cluzel et al [30] and other studies [35], [36] showing a much lower apparent cooperativity.
However, use of this translational fusion resulted in much lower apparent GFP intensities and fewer foci.
We also immunoblotted for endogenous SPAK and OSR1, and observed that these enzymes migrated with a much lower apparent molecular mass, eluting between the 158 kDa and 44 kDa markers.
Interestingly, despite SPAK and OSR1 binding to RFXV motifs of WNK isoforms [ 15, 25], we observed that SPAK as well as OSR1 eluted at a much lower apparent molecular mass region of the gel filtration column than WNK isoforms.
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69 This appears to support conclusions in a recent Ontario study 68 that the actual availability of psychiatrists in Toronto is much lower than the apparent availability since many psychiatrists care for relatively small number of high-income patients.
Examination of the absorbance titration curves indicates that at pH values much lower than the apparent pK (~7.2) some 335 nm is still present and that at pH values higher than pK some 420 nm absorbing species remains.
This apparent Tm is significantly higher than the one found for CYP101A1 (P450cam, Tm = 54°C, measured by differential scanning calorimetry (DSC)) but also much lower than the apparent Tm reported for CYP119 (Tm = 91°C, measured by DSC), a hyperthermostable cytochrome P450 monooxygenase from S. solfataricus (McLean et al. 1998).
In cases with poor DNA quality, this is also immediately apparent from much lower or even missing peaks.
The cluster concentration is much lower in exons and no apparent positional preference observed.
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