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The advantage of using thin slabs with small volume of analyte solution may be hampered by the much longer binding kinetics needing the auxiliary mixing devices.
However, the wrapped junction presents a much longer binding range than the bare junction, while the ''force enhancing point'' locates at 0.38 nm (the interaction point after the first valley).
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The largest difference between our AnkB/AS structure and the reported ANKRA2/HDAC4 and RFXANK/RFX5 structures is that AnkB uses a much longer target binding groove than the other two ankyrin repeats in binding to its targets.
The enzyme structure reveals that the Cel6B enzyme has a much longer substrate-binding site than its fungal GH6 counterparts.
Interestingly, element III found in the present study was much longer than the binding sequence of any Broad Complex isoform.
But the long CFs reported here are likely to play a role other than RNA protein recognition sites as they are much longer than known binding sites.
Because the UCRs are much longer than usual protein binding motifs, it is possible that multiple binding motifs are present in a single UCR.
It is important to remember that the SHCS data are void of any mass-transport effects, as the diffusion of molecules occurs at a much longer time scale than the binding kinetics observed in this study.
These sonicated fragments are often much longer than the actual protein-binding site and, thus, the sequence tags of the ChIP-DNA distribute in broad regions around the actual binding sites.
In GI.2 FUV the much longer P-loop reaches the HBGA binding interface and two residues (G343′ and G245′) are involved in the formation of the Le epitope binding site (Kubota et al., 2012).
One possibility is that SR binding is actually much longer, and FRAP data on arrays is dominated by non-specific interactions and is not capable of detecting the small percentage of receptors which might be stably bound.
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