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There are no telephones, much less cell phones or computers, and not a TV in sight.
In any case, we observed much less cell death when a lower S1P concentration (3 µM) was used.
However, the simulation results showed that there was much less cell variability in the timing of inclusion formation than seen in the experimental data.
As opposed to spheroids grown from single cells in agarose gel (Fig. 6A, Condition 3), the transferred spheroids did not have time to accumulate significant levels of solid stress, and they had much less cell death (Fig. 6A, Condition 2).
Although vacuolin-1 is at least 10 times more potent than CQ in inhibiting autophagy (Fig. 2 A; Fig. S7), vacuolin-1 showed much less cell toxicity than CQ (Fig. 2 B; Fig. S8).
Mutants NPDF-A, N316AF320A, N316D, N316Q, and F320I all showed much less cell surface levels compared to the wild type ABCG1, even though the protein levels in whole cell lysates were similar.
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In practical FRET measurements, this resulted in much less cell-to-cell variability and it allowed collection of meaningful FLIM data in prolonged time-lapses.
Unlike ChIP experiments carried out using embryos, which are composed of many different cell types, these experiments analyze the binding of these transcription factors in two tissues with much less cell-type complexity.
It has been shown that NK cells are abundantly present in the tissue of preneoplastic cervical lesions but much less cells have been detected in cervical squamous cell carcinomas [ 15, 16].
At both pH values, despite the presence of 10 mM Gln, there were much less cells in the presence of the glutaminase GLS1-specific inhibitor 968 compared to its absence.
On 44 hours (after addition of serum)), the cell started to proliferate and spread; cells cultured on P-COL substrate adopted elongated morphology, while those cultured on T-COL exhibited a more polygonal shape; in comparison, much less cells adhered to TCPS, and cells cultured on TCPS were poorly spread, implying immature cellular cytoskeleton assembly.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com