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Recently several genomes of different MOB have been sequenced by the Omega consortium (Khmelenina et al. 2013; Kits et al. 2013; Khadem et al. 2012; Stephenson et al. 2017) and thus a much larger gene dataset is now available to design new primers to potentially cover a larger methanotroph diversity.
Much larger gene changes were validated in a number of novel genes as exemplified by TMEM176A, which showed 35-fold enrichment in the cortex and 30-fold enrichment in hippocampus of FRL animals relative to FSL.
The cell lines of this study were very similarly classified using a much larger gene set (data not shown).
Our experiments consider much larger gene sets and a significant number of essential genes are separated out from non-disease genes.
On the other hand, many patterns with low support observed in the study of Mutanen et al. [ 9] with dense taxon sampling, but lower amount of molecular data, have subsequently been obtained in other studies with much larger gene sampling.
Random PCR shows that they contain cDNA clones of up to 2000 bp but the 5′ end of a much larger gene could be amplified, indicating that even large cDNA clones are represented in the libraries.
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This is of particular importance for other organisms that have much larger genes and introns, such as mammals.
These include cytotoxin gene fragments, remnants of a much larger cytotoxin gene which is thought to have been similar to those still found intact in C. muridarum [ 25].
Meg3/Gtl2 is the founding member of a much larger imprinted gene region that has been implicated as having parent of origin effects in three mammalian species.
This exchange is much larger than gene conversion tract lengths in Drosophila (Hilliker et al. 1994; Schaeffer and Anderson 2005).
Sireviruses also have a much larger gag gene compared to classic retrotransposons, which is characterized by a central RNA-binding CCHC zinc knuckle and a downstream predicted coiled-coil domain [ 13, 18].
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