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Supplementation with FGF resulted in fewer colonies in F12 and LG media, but much larger colonies were formed.

The LIB-SEL population displayed the highest growth rate in adherence, and formed much larger colonies in soft agar compared to LIB-UNS cells (p < 0.0025).

Interestingly, GM3S knockdown in 4T1 cells significantly reduced the number of colonies and slightly decreased the colony size, whereas 67NR cells over-expressing GM3S formed much larger colonies than did control cells, and the number of colonies was slightly increased.

These cells did not display decreased proliferation when compared with control cells and shD26-1 (Supplementary Figure 4b); however, when grown in soft agar, the shSHP-1 cells made much larger colonies than the control further indicating a lack of a proliferation defect and very different phenotype than DUSP26 inhibition (Supplementary Figure 4c).

As gannets typically remain on the continental shelf to forage (location of preferred prey; Fletcher and Tregonning, 1992), these much larger colonies are likely to have established mutually exclusive foraging zones stretching into Bass Strait, potentially restricting the foraging range of individuals from the Pope's Eye colony (Fig. 2).

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Fels also agreed to finance a much larger colony at Hollesley Bay in Suffolk, to be operated as a government scheme under the Local Government Board.

When the increased foraging mortality was applied to foragers at the food patch on each successful foraging trip, the impact on the colony was much larger (colony reduced down to almost 4,000 bees in June), as the mortality was applied many times per day and background mortality is higher than for in-hive life stages.

In support of this hypothesis, murine SP cells give rise to all three mammary epithelial cell lineages when transplanted to cleared mammary fat pads [ 21, 35]; moreover, human SP cells form much larger branching colonies in matrigel than do non-SP cells [ 33].

Furthermore, the colonies formed by the CCN6-deficient cells were much larger than the colonies formed by the control cells.

The colony formation assay results displayed that the Hep2 cells transfected with miR-27a mimic showed much more and larger colonies compared to the control groups, but the cells transfected with miR-27a inhibitor revealed much fewer and smaller colonies compared to the controls.

As showed in Figure 1C, HepG2 and Huh7 cells transfected with 20 nM miR-138 mimic displayed much fewer and smaller colonies (218 or 161 colonies) compared with NC duplex transfected (783 or 729 colonies) and non-transfected cells (756 or 692 colonies), but cells transfected with 20 nM miR-138 inhibitor displayed much more and larger colonies (1238 or 1349 colonies).

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