Expression of variants I and II was relatively high (although much higher for variant I), which made it possible to reliably and precisely assess its levels by quantitative PCR.
Further, the sample sizes required to map rare variants are much higher than for common variants, unless those rare variants have quite large individual effects.
These results emphasize the conservation of variant function between the two species, and indicate that a much higher number of variants can be analysed in zebrafish than is feasible in the mouse.
The much higher number of variants available for testing in the HapMap 2 data set requires a more stringent threshold to prevent overfitting of GWAS models.
If the genome of the sample is very different from the reference sequence, not only a much higher number of variants will be detected, but many reads will not be mapped altogether due to a limited number of mismatches allowed by the mapping algorithm.
The longer Aβ42 variant has a much higher propensity to form aggregates. Genetic studies identified mutations in three genes that cause familial forms of AD (FAD): APP, presenilin-1 (PS1), and presenilin-2 (PS2) [ 12].
Number of missense mutations identified from transcriptome sequencing (67,641 variants) were much higher than from exome sequencing (30,649 variants).
Our method was able to confirm a much higher percentage of structural variants and did not rely on any previous information to identify potential structural variants to be identified and validated.
Sequence data have a much higher density of genetic variants and a different allele frequency distribution, and can have higher genotype error rates.
Only one mutation in exon 1, a transition G128A that alters aa 43 R to K, was found and predicted to be tolerated, so the number of natural variants was much higher than that obtained with induced variation.
