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The results showed that the GhEF1A8 gene is highly expressed in different organs of cotton plants, and showed much higher transcript levels in stems and leaves.
The DEF2 plants showed much higher transcript levels, which were on average 450-fold higher compared to WT.
In B. distachyon, the CSLH gene showed a much higher transcript level and played a prominent role in (1, 3; 1, 4 -β-D-glucan synthesis [ 64 -β-D-glucan
Cluster 2 with 14 members, including 8 members of the VviERF6 clade, had much higher transcript abundance in the skin with a sharp peak at 23.2 °Brix.
The 14-d-old seedlings of selected overexpression lines showed a much higher transcript level as compared to wild type (Col).
Orthologs of RIN and SPL (squamosa) tomato transcription factors, which are known to be very important fruit ripening trancription factors [ 63, 64], were at much higher transcript levels in the skin and decline with °Brix level (Additional file 2).
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Based on conserved amino acid stretches that are indicative for gliadins from the homoeologous loci Gli-2A and Gli-2B[ 11, 36] expression from the Gli-2A locus was estimated to be much higher (normalised transcript abundance of 76 99 per plant) compared to expression from Gli-2B (normalised transcript abundance of 1 20 per plant).
A recent study in mammals, which accounted for methodological and technical issues of previous studies, found a much higher correlation between transcript and protein levels (Li et al. 2014); transcript levels explained at least 56% of the differences in protein abundance.
The qPCR results confirmed the results of the mRNA-seq experiment with the group B gene VvSTS48 showing much higher levels of transcript accumulation than VvSTS genes in groups A (VvSTS6) and C and (VvSTS16) respectively under all stress treatments.
This is associated with a much earlier (and higher) transcript accumulation of most XTH genes than when treated with 0.5 µL L−1 ethylene, suggesting a strong correlation between ethylene, XTH gene activation and cell separation in the AZ.
Pulp cells also have a much higher set of transcripts involved in carbohydrate metabolism, but a lower set of transcripts involved in lipid, amino acid, vitamin, nitrogen and sulfur metabolism than in the skins [ 4].
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