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Much higher sequencing depth is needed to detect SNVs at less than 5%% fraction.
In addition, the larval data are of much higher sequencing depth (averaging 34.5 million reads per sample compared with 15.6 million reads per sample for adult astrocytes).
We calculated that theoretically up to 90% of the current 18,000 genes would be detected across all 35 lanes with this much higher sequencing capacity.
The markedly higher amount of duplicate reads in the RiboZero libraries compared to the RiboMinus libraries can partly be explained by the much higher sequencing depth of those samples.
We noticed that the methylome data generated for ADS-iPSCs are of much higher sequencing depth compared with the other two methylomes (28Xs read coverage on average for ADS-iPSCs, 20Xs for ADS and 22Xs for ADS-adipose).
The identified piRNA clusters includes more than 98% of the 182 known piRNA clusters [ 1], and our much higher sequencing depth allowed us to identify many more clusters expressed at lower levels.
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We combined DC-81 and an indole carboxylate moiety to synthesize a hybrid designed to have much higher sequence selectivity in DNA interactivity.
A third Alu element is found downstream of the two distal Alu elements belonging to the cassette that shows a much higher sequence variation and distinct subgroups within the alignment.
The overall conservation of the Dact4 protein sequences was low, but several recognsizable motifs showed much higher sequence similarity.
Indeed, comparison of 19 BARE1 LTR deposited at TREP showed much higher sequence variability in that region (data not shown).
PcR box of vertebrates shows much higher sequence homology with PcR box of cnidarians compared to that of C. elegans.
More suggestions(15)
substantially higher sequencing
significantly higher sequencing
much higher pay
much lower sequencing
much longer sequencing
much higher failure
much deeper sequencing
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much shallower sequencing
much higher speed
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much higher end
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