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In vitro cellular uptakes of such NPs were investigated with confocal laser scanning microscopy (CLSM), which demonstrated much higher internalization of the folate-decorated QDs-loaded PLA-TPGS/TPGS-COOH NPs by MCF-7 breast cancer cells which are of over-expression of folate receptors than the cellular uptake by NIH 3T3 fibroblast cells which are of low expression of folate receptors.
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Generally, we found that the silica-coated CdSe QDs show much higher cellular internalization efficiency, possibly because of cytocompatibility of silica shell.
While poly(G46- b-P13) at 5 N/P was the poorest polyplex formulation to promote cell entry (25% Cy5-pDNA positive cells), the internalization was much higher at a higher N/P ratio (10 N/P) as 75% of HeLa cells were positive for Cy5-pDNA.
Their internalization rate is much higher in MCMV-infected fibroblasts due to the decreased recycling rate, suggesting that MCMV infection inhibits recycling from juxtanuclear endosomes.
Although electroporation allows high internalization efficiencies to be achieved, the amount of non-internalized fluorescent molecules that remain in cell suspension after electroporation is typically much higher than the amount of internalized molecules.
The international price would be much higher.
Interestingly, the internalization of the rod-shaped, high-aspect-ratio nanoparticles occurred to be with much higher efficacy than that of nanoparticles with an equal aspect ratio.
(Much higher).
How much higher?
They were much higher.
Production was much higher.
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CEO of Professional Science Editing for Scientists @ prosciediting.com