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An alternative to the approach of chemical modification of the coating of a nanoparticle with NIR dyes in order to obtain a fluorescent agent is the encapsulation of the fluorophore within the particle coating) which has been shown to result in a much higher fluorescence signal and photostability of the fluorescent dye than a surficial dye conjugation) [ 8, 27].
However, embryos exposed to the temperature step within 20 minutes after fertilization have a much higher fluorescence background (Figure 4E) in comparison to embryos that developed at uniform temperature (Figure S9), suggesting that Bcd not trapped within nuclei or the energid was at least partially mixed, and that the mixing observed was not reversible.
A much higher fluorescence intensity was observed in blood vessel endothelium than in adjacent structures such as fibro-connective tissue of the lamina propria and striated muscle.
The close location of the nucleobase (rigid ethynyl bridge) seriously affected pyrene spectral behavior, with UP being nonfluorescent and AP with much higher fluorescence quantum yield.
In our profile, fluorescence showed a decreasing intensity in high fragment lengths, except for the 318 bp peak containing 6 co-migrating fragments which exhibited much higher fluorescence intensity than the peaks of similar length.
We subsequently conjugated a commercial fluorophore, BODIPY FL, to the N1-position of granisetron and obtained high-affinity probe 6, which had much higher fluorescence intensity, and was used to visualize recombinant 5-HT3ARs in mammalian cells.
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Among the novel composites, N-doped CQDs or nitrogen/sulfur co-doped CQDs (N, S-CQDs) demonstrated much high fluorescence quantum efficiency or photocatalytic activity than that of pristine one [11, 12].
As the fluorescence of 19TεC is relatively low in duplex DNA, we also prepared a 19AεA substrate that has much higher basal fluorescence.
Based on an optimized polymer mediated transfection procedure using a luciferase assay and confocal microscopy, these two poly(disulfide amine)s induced up to 16-fold higher luciferase expression and much higher green fluorescence protein expression than branched poy ethylenimine) (bPEI, 25 kDa) in primary myoblasts.
The relative lack of membrane localization of Ae2-GFP could not be attributed to low expression levels, because increasing the amount of injected Ae2-GFP cRNA by 2 3 fold consistently produced the same distribution pattern in MII eggs, despite much higher overall fluorescence (not shown).
Shade leaves exhibited a much higher chlorophyll fluorescence yield than sun leaves.
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