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In principle, ULF modulation of energetic electron precipitation, inducing an additional periodic ionization of the lower ionosphere, can cause periodic TEC variations with much higher depth than geomagnetic field variations (Watson et al. 2015).
For WGS and exome data, high sensitivity and specificity could also be achieved, but only at much higher depth of coverage.
The literature thus supports our ability to identify known pathogens using a 16S rRNA amplicon sequencing approach, which look at the entire microbiota of each site with much higher depth coverage and much lower selection biases than previous studies.
We confirmed some previous findings while not others, in addition to identifying novel differentially methylated CpGs at these genes of interest, due to the much higher depth of sequencing coverage in BisPCR compared to prior array-based approaches.
The PCR amplification (instead of exon capture), longer read lengths on the MiSeq platform, and the much higher depth of coverage provided a strong method of validation for SNVs and indels.
Exon capture and sequencing at high depth with one platform can yield a much higher depth of coverage in most exonic regions, whereas WGS offers a more uniform and comprehensive coverage that appears to cover regions missed by exon capture and sequencing.
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The distribution and diversity of the taxa identified from the three enrichment cultures indicates that certain organisms have been sequenced at much higher depths.
Although the optimal number of reads per sample cannot be easily determined without taking into account several factors of an experiment, most studies agree that a much higher sequencing depth is required for the identification of alternative splicing events compared with DE of genes, and that the depth of sequencing has more impact on low rather than on high abundance transcripts [ 22, 23].
However, the average depth of coverage was above 100, and for RNA-seq data, the preferentially expressed genes will have a much higher read depth.
Much higher sequencing depth is needed to detect SNVs at less than 5%% fraction.
Although the alignment of the short reads showed much higher average depth, the coverage fluctuated throughout the chloroplast.
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