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The engineering strain X4, which had much higher copy number and could secretively express HBscFv-IFNγ, was screened from transformed X33 by qPCR.
There are much higher copy numbers (∼175,000 copies) in mature oocytes [4], which may reflect that a sufficient mtDNA reservoir is required to ensure distribution of mitochondria to the cells of the early embryo.
It has long been a puzzle as to how these deleted elements are able to amplify to a much higher copy number than their parents.
Similarly, Caspar (and other CACTA) transposons contain regions with much higher copy numbers than the overall average for the whole element, because of the presence of large arrays of tandem repeats.
Some are capable of achieving much higher copy numbers (Charrier et al. 1999; Lepetit et al. 2000; Hikosaka and Kawahara 2004; Macas et al. 2005; Ray et al. 2005; Remigereau et al. 2006).
Here, we show that the centromeric repeats in T. brucei chromosomes are present at much higher copy number than predicted, with an organisation that is more typical of centromeric domains in higher eukaryotes than realised.
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The frequency distribution of raw reads in each re-sequenced genome (Fig. 7) suggested that this LTR-RT is presented in each genome in much higher copies than surrounding sequences.
It gives me an impression of a much high copy number of such inserts in grapevine compared to other completely sequenced genomes.
A much higher virus copy number was observed in line 7 chickens indicating varying levels of virus replication [ 14].
Among the 33 cases with paired tissue, 8 (24.2%) showed a much higher gene copy number than normal controls (Fig. 3a).
Particularly striking are two superclusters, much higher in copy number than any cluster identified in Lotus (Fig. 3b, note that the scale for resistance gene homolog distributions in Medicago and Lotus are different).
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