This leads to a much higher assay-to-blank ratio for the tandem mass spectrometry assay versus the fluorometric assay and to a much more accurate assessment of enzymatic activity in the low-end range.
PPP rates were much higher when assayed in 20 mmol/l compared with 2 mmol/l glucose, regardless of the growth medium glucose level, and growth in high glucose and assay in high glucose evoked the highest PPP activity.
This means that the protective effect of S. aureus on STS-induced cell death, determined using whole cultures would be much higher if the assay was restricted only to those infected macrophages.
For the comparison of the assays detecting A(H3N2) antibodies, laboratories D and K also had much higher 3-day MN assay titers than 2-day ELISA MN assay titers.
With a detection limit of 1 PFU/ml, RT-LAMP assay exhibited much higher sensitivity than PCR fluorescence probing assay, as well as virus culture.
The IC50 of positive control for α-glucosidase inhibitor (acarbose) is found much higher in the present assay which is similar to many previous literatures (Shinde et al. 2008).
In addition, the throughput by a single operator is much higher for the multiplex assay than for ELISAs: in our set up 84 patient samples can be processed for nine analytes within four hours under highly standardized conditions.
For comparison of assays detecting A(H5N1) antibodies, laboratory H had much higher 3-day HA MN assay titers than 2-day ELISA MN assay titers (Table 4).
This could explain the fact that only at much higher concentrations in these assays did geminin prevent religation, but not cleaving activity, of TopoIIα.
Overall, the Wantai assay found a much higher seroprevalence of anti-HEV antibodies compared with the WRAIR assay, using the same serum.
This is mainly because the standard deviations are much higher in the β-galactosidase assay.
