First, in most cases the fold change was much greater by quantitative RT-PCR than by array, and second, there was an evident dose effect in six of seven genes by quantitative RT-PCR which was not apparent by array.
Finally, we found only 2 reviews in which quantitative changes much greater than 50% led to altered conclusions.
With the advance of live imaging techniques [20], [21] it will become possible to study cell pool dynamics in the SAM with much greater detail and thus allow quantitative modeling of cell behavior with high spatial and temporal resolution.
RT-PCR has often been subsequently used to verify the results of microarray data, since it offers much higher sensitivity, specificity, reproducibility and a greater quantitative dynamic range.
Indeed, because there was virtually no expression of PUMA in control cells, presumably due to p53 mutation, there was a much greater fold increase in expression as seen by quantitative RT-PCR.
A model with much greater detail would be better suited to providing a quantitative assessment of the issues being explored here, and we believe we can construct one from the building blocks introduced here.
However, when the quantitative relationship was not available, there were much greater estimation errors.
Furthermore, Hard (2004) showed that, in chinook salmon (Oncorhynchus tshawytscha), accounting for correlated characters in a multivariate quantitative genetic model yielded responses to selection much greater than estimates based on single-trait approaches.
Such action is not a substitute for more quantitative easing; but the stimulative impact would so much greater, with far fewer side effects.
While not a quantitative measure, the macroarray was able to detect certain fungi with much greater sensitivity than T-RFLP.
