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We've seen the U.S. budget deficit go from ten and a half percent of GDP to four percent of GDP in a much faster frame of time than anybody could have thought.
To overcome this limitation, we imaged oocytes expressing GFP-Stau at high magnification with much faster frame rates (2 4 frames/s) using a sensitive wide-field deconvolution microscope.
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"In contrast, we are seeing strong growth in the Australian market and are targeting a much faster time frame — the customer response to the Iconic brand and experience has been far stronger we would have imagined.
You should also keep in mind that trees planted from seeds could take 8 to 10 years to produce fruit, while a transplanted sapling will produce fruit in a much faster time frame.[3].
This fact can be observed from Table 3, where Avg-Feature is 6 times faster than MSM and 40 times faster than AHM with 4 frames and even much faster with 16 frames.
However, if the diffusion rate is much faster than the frame time, even at 1000 frames/s, then the signal would give the center position and the spot would not noticeably broaden.
Although the results presented here are taken with video rate (30 Hz) acquisition, much faster exposure times and frame rates are possible at the cost of increased damage to the animal [ 2].
10.7554/eLife.05413.028 Author response image 3. DOI: http://dx.doi.org/10.7554/eLife.05413.028 This result shows that the diffusion rate is comparable to the exposure time of 0.56 ms. For complete averaging of the position one would indeed require a much faster diffusion than the frame time, which is not the case here.
Instead of estimating absolute B-factors for each movie frame, the alternative approach merely estimates 'relative B-factors' that describe how much faster (or slower) the signal in reconstructions from individual frames drops with frequency compared to the average reconstruction from all movie frames.
The following explanation was added above the formula: "Instead of estimating absolute B-factors for each movie frame, the alternative approach merely estimates "relative B-factors " that describe how much faster (or slower) the signal in reconstructions from individual frames drops with frequency compared to the average reconstruction from all movie frames.
Moreover, by ensuring that the system derived from our approach is always a tight frame, our approach also runs much faster than other over-complete dictionary based approaches with comparable performance on denoising.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com