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Exact(5)
There were differences in both the kinetics and the pattern of the exchange reaction, with the p53 and p63 tetramers exhibiting much faster exchange kinetics than p73.
At the proximal end, paxillin and vinculin display much faster exchange rates than at the distal end (Figure 5).
The glycerol facilitator (GF), a transmembrane protein responsible for transporting water and glycerol, was probed with HDX MS. One of the transmembrane helices, TM7, displayed much faster exchange kinetics relative to the other helices suggesting that TM7 is less stable than the other helices in order to aid transport of molecules across the membrane.
Proteins with much slower exchange rates can still be coeluted with the NPs in F4 and identified in the after-column NP collection with significant abundance; while those with much faster exchange kinetics are lost in the column and not detectible in the collection.
With this one exception, peptides of the C-terminal side of the methylation subdomain (the N-terminal side peptides exchange too quickly to measure) exhibit two changes in the high-density kinase-activating state relative to the low-density kinase-inhibited state: approximately half of the CF exhibits much faster exchange (complete in 3 min), and the other half exhibits 4-fold slower exchange.
Similar(55)
Second, the model should be able to unify the apparent slow proton exchange observed by NMR in certain proteins with the much faster chemical exchange indicated by FCS (compare, for example, Figure 3C,D with Figure 5A).
For reference, the data in Figure 3A were collected with a 300 MHz NMR spectrometer, which makes the inverse of the frequency difference between the basis states approximately 1 ms. Systems undergoing much faster chemical exchange will appear as a single peak, while those exchanging much slower would result in two peaks.
In accordance with recent theoretical findings, much faster surface exchange (∼4 times) and diffusion (∼10 times) were observed for the tensile strained films compared to the compressively strained films in the entire temperature range.
Applying this procedure to the two profiles in Figure 3 yields k* = 9.67 × 10 11 cm/s, D* = 8.0 × 10 16 cm/s for LSC/LAO and k* = 5.83 × 10 10 cm/s, D* = 1.9 × 10 14 cm/s for LSC/STO and thus a much faster oxygen exchange and diffusion in the tensile LSC film at 400 °C.
On the other hand, non-thermo-sensitive oligomers such as PAA and PEG are characterized by much faster diffusion and exchange kinetics of proximal water at the two simulated temperatures compared to PNIPAM.
These result could be explained by the fact that diselenides (selenocystamine) are much stronger electrophile than disulfides (cystamine), and also, the rate of selenol/diselenide exchange is much faster than the corresponding rate of thiol/disulfide exchange (Hondal et al. 2013; Pleasants et al. 1989).
Related(17)
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