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The enzyme had also a preference for ribonucleoside over deoxyribonucleoside triphosphates as rATP was a much better substrate than dATP.
The much greater increase in secondary siRNA accumulation caused by the dcl4 mutation in lines carrying both transgenes than in those having the hairpin locus alone suggests that the GUS sense transgene transcript is a much better substrate for RDR6-dependent production of siRNAs than the hairpin transcript.
However, Tl+ is a much better substrate than K+, which is not transported at all.
The (1S -anti-diol epoxide was a much better substrate than the (1S -anti-diolr, as was true also for (1S -anti-diolR)-trans-1,2-dihydrodiol.
C5-HSL was a much better substrate, exhibiting lower KM values (800 μM) and higher kcat values, leading to a 4 order of magnitude increase in kcat/ KM values.
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Thus, we cannot exclude that much better substrates with higher coupling efficiencies exist for CYP154H1 since the natural substrate of this enzyme is not known.
In this case Class 1 peptides (with Arg/Lys residues both N- and C- terminal to the phosphorylated residue) were much better substrates than peptides based on other substrate classes (Fig. 1b).
In this report, we demonstrate that, in vitro, SAM is a much better CqsA substrate than LAB.
(18) Together, these results suggest that SAM is a much better CqsA substrate than LAB and therefore that SAM may play an important role in in vivo CAI-1 synthesis.
The film-cohesion for Ti/TiN multilayer film on the nitrided substrate was much better than that on the substrate without nitriding treatment.
However, all of the compounds tested with modifications at the 7-position were much better inhibitors than substrates.
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