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The assembly of the CF3 metagenome yielded high coverage and significant BLASTn hits to the genome of H. influenza prophage Mu (Figure S5).
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Kinetic assays confirmed that the AOX-driven reaction has proceeded enormously fast compared to the DhaA-mediated dehalogenation using 1.5 U AOX and 6.4 mU DhaA (Figure 4B).
Dissociated spheres re-exposed to serum rapidly expressed initial levels of WT and MU transcripts (Figure 8G).
A total of 156 units were recorded (53 SU and 103 MU). Figure 1 shows several example units where we measured SRFs for several different probe tone frequencies.
‡ designates mutations that were combined to re-establish a salt bridge between beta and mu (See Figure 5 ).
Two MUs were located on the surface of hemoglobin forming a disulfide bond with β-Cys93 (MU-1/MU-1′; Figure 3B and Supporting Information Figure S3).
Two related mini-Mu transposons (Figure 1C) were constructed using standard PCR and cloning procedures.
We also constructed two selectable marker-containing mini-Mu transposons (Figure 1C).
In the calculations that follow, we first consider (mu =9.2). Figure 1 illustrates the slow flow in the neighborhood of the folded node (black dot) on the critical manifold (gray surface), which is projected onto the ((x,y,z -space.
The map has twenty-one MUs (Table 1, Figure 8).
However, many populations also showed substantial overlap consistent with non-significant MUs (e.g., Figure 3C,F and Additional file 1: Figures S2, S3).
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