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Samples were then demagnetized with backfields of −100 and −300 mT using the VSM3900.
The calculated periodicity of 60 mT using the relation [23], B_{focus}=frac{sqrt{2}hbar k_{F}}{eL} (1). is in good agreement with the experimental result.
Some samples were demagnetized using both the in-line demagnetizer and DEM-95; first they were demagnetized up to 100 mT using the in-line demagnetizer, and then further demagnetized from 110 to 150 mT using DEM-95.
The TASH score is transformed into a probability for MT using the following logistic function: p = 1 / 1 + exp 5. 4 - 0. 3 * TASH.
The IRf PELDOR spectra were also recorded close to g⊥ at 334 mT using the same τ1 and τ2 values as for the regular four-pulse ELDOR (a).
Immunohistochemical staining of randomly selected, formalin-fixed and paraffin-embedded normal and malignant nasopharyngeal tissues were analysed for the expression of MT using the commercially available E9 antibody directed against MT I and MT II isoforms.
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For this purpose, an iterative frequency-domain receiver based on the IB-DFE concept [19] is considered, as illustrated in Figure 3, allowing an efficient separation of the signals associated with different MTs, using the same physical channel that is able to take full advantage of macro-diversity effects.
To show that native dynein slides MTs using the proposed mechanism, the authors could use 'minimal-dimeric-motor' recombinant yeast heavy chains dimerized natively.
Because the signal of mCherry-labeled MTs is much weaker than that of GFP and undergoes rapid photobleaching, it is extremely hard to detect in trichomes due to their three-dimension geometry; therefore, to ensure an accurate comparison, we further observed KCBP and MTs using the GFP-KCBP-expressing line and the GFP-TUB6-expressing line, respectively.
However, since all the MTs use the same substrate, SAM, up and down regulation of a particular MT will affect the flux in all MT reactions, i.e. it is hard for the cell to regulate each of MT fluxes independently.
The MT uses this information to generate a DSMS.
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