Modification of pIX may destabilize the capsid structure; this possibility was assessed by comparing the thermostability of Ad-tGFP-pIX-MT to a control Ad-CMV-EGFP virus containing the wild-type pIX and to an Ad-IX-EGFP virus incorporating the enhanced GFP (EGFP) protein as a fusion to pIX.
Spindles consist of two centrosomes organizing a set of MTs to a bipolar apparatus.
Overexpressed EB-MMG strongly displaced EB1 from MTs to a diffuse cytoplasmic and nuclear localization identical to EB-MMG staining in these cells.
In contrast, segmentation of non-K-fiber areas resulted in distinct, particulate density and translating MTs to a non-K-fiber area meant that globular density, which passed through the MTs, was detected.
Another test we employed early on to give us confidence in mesh detection was to move the segmented MTs to a non-K-fiber area and do the mesh detection.
In contrast to biasing MT directionality across the growth cone by Wnt3a (Purro et al., 2008), we show that Wnt5a changed the array of splayed MTs to a bundled organization such that dynamic MTs reoriented in the direction of increased axon outgrowth.
A similar but unaltered card, the 'Jumbo Wagner', also has a NM-MT grade quality for the majority of the card, but the lower quality edges have not been trimmed, lowering it from NM-MT to an overall grade of PSA 5 – one of only three (along with Mastro's trimmed card) rated PSA 5 or better – sold at a 2013 auction for $2,105,770.50.
We encouraged MT to keep a log of when he experienced pain to better understand the pattern of occurrence.
Imports from Sardinia fell from 18,200 MT to 13,100 MT (a fall of 28.3%).
Finally, Dr Aschner examines the ability of MT to protect against a specific toxicant, methylmercury, in the CNS.
