BtubA-BtubB heterodimer is an essential intermediate in protofilament assembly.
Isothermal titration calorimetry gave a value of 0.78 μM for the dissociation constant of the FtsZ-SulA complex, similar in magnitude to the 0.72 μM critical concentration of FtsZ protofilament assembly at steady state.
Snf7 assembles into spiraling protofilaments on membranes, presenting two challenges: (1) to characterize the conformational state of Snf7 building blocks; and (2) to determine the protofilament assembly from these structural elements.
The elaborate disc architecture is defined by novel proteins and complexes that decorate almost all disc MT protofilaments, and vary in composition and conformation along the length of the MTs.
In the text, the authors present the monomeric crystal structure of intra-protofilament point mutant S283D as evidence the double-protofilament assembly is disrupted.
Tyrosine kinases have been implicated in the regulation of MT nucleation, assembly, and stability.
Repeats in the mt genome assembly were detected using RepeatMasker in default mode (http://www.repeatmasker.org).org
By this principle, over several rounds of MT assembly and disassembly, all MTs converge into one coherent super-structure at the position of highest RanGTP concentration.
The above results clearly suggest that MT assembly starts with the formation of protofilaments made of longitudinally bound tubulin dimers.
Despite the plethora of such data produced, the molecular mechanisms of MT assembly remain disputed.
