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Subsequently, the complete mt genome was amplified by long-PCR from the genomic DNA in two overlapping regions (~5 kb and ~10 kb, respectively) [ 14].
The complete mt genome was amplified from ~10% (20 40 ng) of the genomic DNA from the individual specimen by long-PCR (BD Advantage 2; BD Biosciences) as two overlapping amplicons (large and small), using the protocol described by Hu et al. [ 14] with minor modifications.
The architecture of the M. oculata mt genome was very similar to that of other scleractinians, except for a novel gene rearrangement affecting only cox2 and cox3.
A putative pseudo-lysine-tRNA in T.paravorax Mt genome was located at 5' end of the genome in the unusually long non-coding sequence (495bp) between the telomere and the large ribosomal subunit.
The final mt genome was found to be 48,463 bp in length.
The mt genome was annotated using the MITOS [ 44] and DOGMA [ 45] webservers.
Primers designed for species-specific PCR, amplifying portions of the mitochondrial (mt) genome, were also suitable for a multiplex PCR.
The complete mt genome is 16,466 bp in length including the typical structure of 22 tRNAs, 2 rRNAs, 13 protein-coding genes and the noncoding control region (CR).
Primer design for amplification and sequencing of an entire mt genome is currently under development.
The repeated tracts in each mt genome were located.
The 119,989-bp cp genome lacking inverted repeats and 65,049-bp mt genome were sequenced.
