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A recent study [ 22] demonstrated that 454 technology is more reliable than a conventional (Sanger) approach for sequencing highly AT-rich regions, suggesting that this approach could enhance long PCR-based mt genome sequencing.
However, for some groups of organisms, particularly invertebrates, there is very limited information on mt genomes, which most likely relates to the inaccessibility of a practical, generally applicable and cost-effective technique for mt genome sequencing.
Complete mitochondrial (mt) genome sequencing is becoming increasingly common for phylogenetic reconstruction and as a model for genome evolution.
The entire mt genome sequencing was easy to complete and could help biologists in identifying the phylogenetic relationship for closely-related species.
LF developed the whole mt genome sequencing strategy, coordinated the experiments and drafted the manuscript.
A similar case of partial mt genome sequencing due to failed PCR amplification was reported for Littorina saxatilis [ 43]).
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The mt genome sequenced for Haemonchus contortus was 14,055 bp in length and was highly AT-rich (78.1%).
Together, the two mtDNA chromosomes represented 91,042 bp, which is the largest mt genome sequenced so far in Glomerales order, while Gigaspora rosea has the largest mt genome recorded in Glomeromycota (97.3 kb; Nadimi et al. 2012).
mt genome data on the sister group of the Volvocales, the order Sphaeropleales, remain represented only by Scenedesmus obliquus (recently recombined as Acutodesmus obliquus; Hegewald and Hanagata 2000; mt genome sequenced by Nedelcu et al. 2000).
The degree of similarity was shown using similarity metric (z score) calculations on ORFs from Mt genomes sequenced in this project with that of previously sequenced T.pyriformis, and T.thermophila.
Of the 41 complete mt genomes sequenced so far, 12 are from bivalves.
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