Several points suggest that this motif may well have functional significance relatively specific to the family 7a MT genes: a) Clusters of multiple, highly statistically significant matches are found within 500 bp upstream of the start codon of the four tested subfamily 7a MTs.
Analysis of the conserved gene cluster in the mt genome is difficult because of limited information regarding mt genomes in trebouxiophycean algae.
Similarly, there was silencing-associated H3K27me3 throughout the gene cluster in Mb, Mt and most examined nonmyogenic cell types as determined by whole-genome chromatin immunoprecipitation/next-generation DNA sequencing (H3K27me3 ChIP-seq; ENCODE/Broad Institute, http://genome.ucsc.edu/).ucsc.edu/
The mt gene order of H. regis has the characteristic LTPF tRNA gene cluster, which can, thus, be confidently considered a molecular synapomorphy of all neobatrachians.
A total of 64 gene rearrangements were identified in the seven evaniomorph mt genomes, including 4 local translocations (within a tRNA gene cluster and without inversion), 32 gene inversions, and 49 long-range movements (some genes were involved in two rearrangement events).
In addition, the four fat body and MT-specific genes were localized in the 5′ portion of the gene cluster, whereas the nine genes expressed mainly in brain-nervous system occupied the other half of the cluster.
The major tRNA gene cluster identified in the F. circinatum, F. verticillioides and F. fujikuroi mt genomes are interrupted by a unique ORF.
The human homolog for this MT gene (Acc nb of the cDNA: AF139682) is present on HSA21, 4.5 Mb centromeric to the cluster and transcribed toward the centromere [ 25].
mitis" gene cluster structure.
(C) Hub-gene network of the magenta gene cluster.
